Blog #9 3/25/24 - 3/29/24

Introduction 

This week I assisted in completing some redolent and redos for the chemical test on Sono. There is about two weeks before the conference. We redid the MR VP test. We also poured more citrate and inoculated them with sono. I also assisted Madison while she completed the UV radiation experiment. 


Methods

Pouring plates

-heat media 

-let it slightly cool down 

-line up plates on the edge of the table 

-slightly open the lid and pour in till you cover the bottom of the plate 

-leave the plate to solidify 


MR VP 

To complete the MRVP we get a tube that contains MRVP broth. Then we needed to inoculate, we had sono as our experimental variable, along with positive and negative control. To begin, turn on your Bunsen burner, then pass your loop through the flame to sterilize it. Then slightly open the plate of bacteria and grab some bacteria. Then grab the tube while using your other hand to take the lid off. Pass the opening of the tube through the fire to sterilize it then put in the loop to inoculate the broth. 


UV Radiation 

I assisted Madison in this procedure, I inoculated the plate while she exposed the bacteria to the UV. 

UV exposure 

(We are exposing six flasks of sonorensis, three are sono grown in tgy and the other three are sono grown in R2B.) 

-nanodrop each flask and normalize to 1

-measure 50µl of each flask and pipet it onto a square of parafilm in the UV exposure machine

-expose the bacteria To a specific energy level for 5 minutes 

-pipette the bacteria into a little tube that contains 540µl of its respective Media 

(This is the part I assisted with) 

-then take the flasks of exposed bacteria and inoculate plates with their respective media. Then place it in the incubator 

( The values of exposure We used were 2500, 3000,3500,4000,5000) 


Results

 Our citrate plates were inoculated With sono and placed in the incubator. The results of the chemical test were negative. The MRVP result came back negative. This means that sono does not ferment by a mixed acid pathway. The UV radiation plates were placed in the incubator 


Results & Conclusion

Next week is the last week before the deadline Week when we do the final edits of our poster and get it printed.q we should be finishing the chemical test and each of us should be finishing our designated poster part.


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