S-STEM Scholar Carime Flores' Blog

Introduction  This week I assisted in completing some redolent and redos for the chemical test on Sono. There is about two weeks before the conference. We redid the MR VP test. We also poured more citrate and inoculated them with sono. I also assisted Madison while she completed the UV radiation experiment.  Methods Pouring plates - heat media  -let it slightly cool down  -line up plates on the edge of the table  -slightly open the lid and pour in till you cover the bottom of the plate  -leave the plate to solidify  MR VP  To complete the MRVP we get a tube that contains MRVP broth. Then we needed to inoculate, we had sono as our experimental variable, along with positive and negative control. To begin, turn on your Bunsen burner, then pass your loop through the flame to sterilize it. Then slightly open the plate of bacteria and grab some bacteria. Then grab the tube while using your other hand to take the lid off. Pass the opening of the tube thr...

  Introduction  The following week I did gram staining on staphylococcus epidermidis . We also discussed the layout of our poster. I will be creating a table that contains a list of all the biochemical tests that will be performed on D. Sonorensis. I also poured plates and helped to prepare some casein plates. I did some research on the procedure of doing a spore stain. Methods I did two different gram stains on a different batch of Staphylococcus epidermidis. plate A was inoculated on March 4th and plate B was inoculated on March 8th. I did a gram stain on both to check if there was any contamination. I followed the standard procedure of doing a gram stain.    Pouring plates -heat media  -let it slightly cool down  -line up plates on the edge of the table  -slightly open the lid and pour in till you cover the bottom of the plate  -leave the plate to solidify  Results There wasn’t contamination in the bacteria plates. Both gram-stains showed ...

  Introduction  This following week was the last week before spring break. This week I inoculated, gram-stain, and made some media. We did two chemical tests, an oxidase test and oxygen requirement test. I also pour plates for the first time  Methods I inoculated and gram-stained by the standard directions. I also made 100 ml of TGY  deionized water -100ml Agar- 1.50g  Tryptone- .3g Yeast Extraction- .3g Dextrose- .1g  pouring plates  We take autoclaved TGY broth and heat it on the hot plate. After heating it let it slightly cool down, to test if it is the right temperature touch the cool-down media to your skin. If the media is ready, line up your empty plates closed at the edge of the table. To pour, slightly open the plate and pour into the media Just till the bottom is covered with media. Then leave the plate at room temperature to Let them solidify.  Oxidase test To perform the Oxidase test, you start by inoculating a plate with bacteria, in ...

  Introduction  The following week, I made some media and did gram-staining on D. caeni and D. sonorensis. My classmates Emme and Bryan did a presentation discussing different chemical tests and their purposes. The plan is to use some chemical tests to characterize Deinococcus Sonorensis. We also had a discussion where we learned the difference between Rt-PCR, q-PCR, and Rt-qPCR.  Methods For the gram staining, I began by getting the bacteria (sonorensis and Caeni) on two separate microscope slides. First, you turn on the flame and sterilize the loop. Then after letting the loop cool, slightly lift the lid of your plate and scoop some of the bacteria with the loop. After spreading the bacteria on the slide finally heat fix it by passing the slide over the flame. Next, you have to complete the steps of the gram stain. You add a couple of drops of crystal violet on top of the bacteria, and after a minute you wash the slide with deionized water. Repeat this step with iodine....